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yes, you could do it with PELTIERS, they are ceramic/alluminum devices that get very hot on one side and cold on the other...you would cool off the hot side with heatsinks and fans while the cold side froze your "assets"
That Peltier design is used in my mini fridge. But I don't think it works very well unless you have long flat sheets (correct me if I'm wrong). It might make for an uncomfortable seat.
You don't need cold seats, you just need air circulation. If you could hook up a small blower or run 2 of the AC vents to the seats, you could use the vent for air.